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Title: Gene delivery efficacy of polyethyleneimine-introduced chitosan shell/poly(methyl methacrylate) core nanoparticles for rat mesenchymal stem cells
Authors: Nuttaporn Pimpha
Panya Sunintaboon
Supharat Inphonlek
Yasuhiko Tabata
Thailand National Nanotechnology Center
Mahidol University
Kyoto University
Keywords: Biochemistry, Genetics and Molecular Biology;Chemical Engineering;Engineering;Materials Science
Issue Date: 1-Jan-2010
Citation: Journal of Biomaterials Science, Polymer Edition. Vol.21, No.2 (2010), 205-223
Abstract: This work investigated polyethyleneimine (PEI)-introduced chitosan (CS) (CS/PEI) nanoparticles as non-viral carrier of plasmid DNA for rat mesenchymal stem cells (MSCs). The CS/PEI nanoparticles were prepared by the emulsifier-free emulsion polymerization of methyl methacrylate monomer induced by a small amount of t-butyl hydroperxide in the presence of different concentrations of PEI mixed with CS. The resulting nanoparticles were characterized by their surface properties and buffering capacity. In vitro gene transfection was also evaluated. The introduction of PEI affected the surface charge, dispersing stability and buffering capacity of the nanoparticles. The CS/PEI nanoparticles formed a complex upon mixing with a plasmid DNA of luciferase. The complex enhanced the level of gene transfection and prolonged the time period of expression for MSCs, compared with those of plasmid DNA-original CS and PEI nanoparticles. Cytotoxicity of CS/PEI complexes with plasmid DNA was significantly low, depending on the amount of PEI introduced. It is concluded that the CS/PEI nanoparticle was a promising carrier for gene delivery of MSCs. © 2010 Koninklijke Brill NV, Leiden.
ISSN: 15685616
Appears in Collections:Scopus 2006-2010

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