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Title: Thai Acanthamoeba isolate (T4) induced apoptotic death in neuroblastoma cells via the Bax-mediated pathway
Authors: Araya Dharmkrong at Chusattayanond
Siriphan Boonsilp
Jitra Kasisit
Atsadang Boonmee
Saradee Warit
Mahidol University
Thailand National Center for Genetic Engineering and Biotechnology
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Dec-2010
Citation: Parasitology International. Vol.59, No.4 (2010), 512-516
Abstract: A Thai Acanthamoeba isolate named AS recovered from a corneal scraping of a keratitis patient was genotypically determined as T4. AS trophozoites were used for studying Acanthamoeba-induced apoptosis in mouse neuroblastoma NA cells during in vitro co-cultivation. The Acanthamoeba-exposed NA cells showed signs of apoptosis including cell shrinkage, nuclear condensation and DNA fragmentation. The effect was confirmed by DNA laddering electrophoresis. Involvement of caspase enzymes and mitochondrial pro- and anti-apoptotic proteins (Bax and Bcl-2) in AS-induced apoptosis was determined. The use of Z-VAD-FMK, a pan-caspase inhibitor, significantly reduced the apoptotic effect, while Bax/Bcl-2 ratio analysis showed a significant increase in the expression of apoptotic proteins in AS-exposed NA cells. These results strongly indicated that apoptosis induced by AS trophozoites is caspase-dependent and is mediated by over-expression of pro-apoptotic proteins in the mitochondrial pathway. This is the first report on the role of Bax in mediating apoptosis induced by Acanthamoeba. © 2010 Elsevier Ireland Ltd.
ISSN: 13835769
Appears in Collections:Scopus 2006-2010

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