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Title: Copro-molecular identification of infections with hookworm eggs in rural Lao PDR
Authors: Megumi Sato
Surapol Sanguankiat
Tippayarat Yoonuan
Tiengkham Pongvongsa
Malaythong Keomoungkhoun
Inthava Phimmayoi
Boungnong Boupa
Kazuhiko Moji
Jitra Waikagul
Mahidol University
Universidade Federal do Tocantins
National Institute of Public Health
National Institutes for the Humanities, Research Institute for Humanity and Nature
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Sep-2010
Citation: Transactions of the Royal Society of Tropical Medicine and Hygiene. Vol.104, No.9 (2010), 617-622
Abstract: The prevalence of hookworm eggs in fecal samples, by modified cellophane thick-smear technique, was found to be 30.0% (61/203 samples) in Lahanam Village, Savannakhet Province, Lao PDR. The hookworm eggs were morphologically heterogeneous, so that identification was confirmed by copro-PCR with specific primers for hookworms and Trichostrongylus spp.; 12 samples were positive for Necator americanus, 19 for Ancylostoma spp., with one mixed infection of both. Sequencing of the Ancylostoma spp. copro-PCR products found A. duodenale, and also the animal hookworms, A. caninum and A. ceylanicum. Moreover, Trichostrongylus spp.-specific copro-PCR revealed a significant rate of infection (43/203; 21.2%). Sequencing confirmed the zoonotic species, T. colubriformis. PCR/sequencing is useful for differentiating parasite species. The positives by the Kato-Katz method, and by the combined hookworm/. Trichostrongylus specific-PCR, were comparable, although the agreement between the two methodologies was only 50%. © 2010 Royal Society of Tropical Medicine and Hygiene.
ISSN: 00359203
Appears in Collections:Scopus 2006-2010

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