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|Title:||Copro-molecular identification of infections with hookworm eggs in rural Lao PDR|
Universidade Federal do Tocantins
National Institute of Public Health
National Institutes for the Humanities, Research Institute for Humanity and Nature
|Keywords:||Immunology and Microbiology;Medicine|
|Citation:||Transactions of the Royal Society of Tropical Medicine and Hygiene. Vol.104, No.9 (2010), 617-622|
|Abstract:||The prevalence of hookworm eggs in fecal samples, by modified cellophane thick-smear technique, was found to be 30.0% (61/203 samples) in Lahanam Village, Savannakhet Province, Lao PDR. The hookworm eggs were morphologically heterogeneous, so that identification was confirmed by copro-PCR with specific primers for hookworms and Trichostrongylus spp.; 12 samples were positive for Necator americanus, 19 for Ancylostoma spp., with one mixed infection of both. Sequencing of the Ancylostoma spp. copro-PCR products found A. duodenale, and also the animal hookworms, A. caninum and A. ceylanicum. Moreover, Trichostrongylus spp.-specific copro-PCR revealed a significant rate of infection (43/203; 21.2%). Sequencing confirmed the zoonotic species, T. colubriformis. PCR/sequencing is useful for differentiating parasite species. The positives by the Kato-Katz method, and by the combined hookworm/. Trichostrongylus specific-PCR, were comparable, although the agreement between the two methodologies was only 50%. © 2010 Royal Society of Tropical Medicine and Hygiene.|
|Appears in Collections:||Scopus 2006-2010|
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