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|Title:||Induction of TNF-α in human macrophages by avian and human influenza viruses|
Chairul A. Nidom
Le Quynh Mai
Institute of Medical Science The University of Tokyo
National Institute of Hygiene and Epidemiology Hanoi
University of Wisconsin Madison, School of Veterinary Medicine
Japan Science and Technology Agency
|Keywords:||Immunology and Microbiology|
|Citation:||Archives of Virology. Vol.155, No.8 (2010), 1273-1279|
|Abstract:||The highly pathogenic avian influenza virus H5N1 is known to induce high level of tumor necrosis factor α (TNF-α) from primary macrophages. However, it is still unclear whether current H5N1 strains also induce high TNF-α production, as most of the data were derived from extinct clade 0 H5N1 strain. Here, we show that current clade 1 and 2 H5N1 strains induce variable levels of TNF-α that are not necessarily higher than those induced by seasonal influenza viruses. The result suggests that hyper-induction of TNF-α in human macrophages is not always associated with a highly pathogenic phenotype. We further tested the contribution of the NS gene segment from H5N1 isolates to TNF-α induction by using reverse genetics. While NS conferred some variation in TNF-α induction when incorporated into an H1N1 virus genetic background, it did not affect TNF-α induction in an H5N1 virus genetic background, suggesting that other viral genes are involved. © 2010 Springer-Verlag.|
|Appears in Collections:||Scopus 2006-2010|
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