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dc.contributor.authorSebastian C. Bhakdien_US
dc.contributor.authorAnnette Ottingeren_US
dc.contributor.authorSangdao Somsrien_US
dc.contributor.authorPanudda Sratongnoen_US
dc.contributor.authorPeeranad Pannadapornen_US
dc.contributor.authorPattamawan Chimmaen_US
dc.contributor.authorPrida Malasiten_US
dc.contributor.authorKovit Pattanapanyasaten_US
dc.contributor.authorHartmut Ph Neumannen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherX-Zell Biotech Ltd.en_US
dc.contributor.otherJohannes Gutenberg Universitat Mainzen_US
dc.contributor.otherInstitut Pasteur, Parisen_US
dc.contributor.otherUniversitats Klinikum Freiburg und Medizinische Fakultaten_US
dc.date.accessioned2018-09-24T09:07:08Z-
dc.date.available2018-09-24T09:07:08Z-
dc.date.issued2010-03-12en_US
dc.identifier.citationMalaria Journal. Vol.9, No.1 (2010)en_US
dc.identifier.issn14752875en_US
dc.identifier.other2-s2.0-77749322698en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77749322698&origin=inwarden_US
dc.identifier.urihttp://repository.li.mahidol.ac.th/dspace/handle/123456789/29251-
dc.description.abstractBackground. Highly purified infected red blood cells (irbc), or highly synchronized parasite cultures, are regularly required in malaria research. Conventional isolation and synchronization rely on density and osmotic fragility of irbc, respectively. High gradient magnetic separation (HGMS) offers an alternative based on intrinsic magnetic properties of irbc, avoiding exposure to chemicals and osmotic stress. Successful HGMS concentration in malaria research was previously reported using polymer coated columns, while HGMS depletion has not been described yet. This study presents a new approach to both HGMS concentration and depletion in malaria research, rendering polymer coating unnecessary. Methods. A dipole magnet generating a strong homogenous field was custom assembled. Polypropylene syringes were fitted with one-way stopcocks and filled with stainless steel wool. Rbc from Plasmodium falciparum cultures were resuspended in density and viscosity optimized HGMS buffers and HGMS processed. Purification and depletion results were analysed by flow cytometer and light microscopy. Viability was evaluated by calculating the infection rate after re-culturing of isolates. Results. In HGMS concentration, purity of irbc isolates from asynchronous cultures consistently ranged from 94.8% to 98.4% (mean 95.7%). With further optimization, over 90% of isolated irbc contained segmented schizonts. Processing time was less than 45 min. Reinfection rates ranged from 21.0% to 56.4%. In HGMS depletion, results were comparable to treatment with sorbitol, as demonstrated by essentially identical development of cultures. Conclusion. The novel HGMS concentration procedure achieves high purities of segmented stage irbc from standard asynchronous cultures, and is the first HGMS depletion alternative to sorbitol lysis. It represents a simple and highly efficient alternative to conventional irbc concentration and synchronization methods. © 2010 Bhakdi et al; licensee BioMed Central Ltd.en_US
dc.rightsMahidol Universityen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77749322698&origin=inwarden_US
dc.subjectImmunology and Microbiologyen_US
dc.subjectMedicineen_US
dc.titleOptimized high gradient magnetic separation for isolation of Plasmodium-infected red blood cellsen_US
dc.typeArticleen_US
dc.rights.holderSCOPUSen_US
dc.identifier.doi10.1186/1475-2875-9-38en_US
Appears in Collections:Scopus 2006-2010

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