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Title: Identification and differentiation of human schistosomes by polymerase chain reaction
Authors: Naoko Kato-Hayashi
Masashi Kirinoki
Yukio Iwamura
Tamotsu Kanazawa
Viroj Kitikoon
Hajime Matsuda
Yuichi Chigusa
Dokkyo Medical University
Ibaraki Prefectural University of Health Sciences
University of Occupational and Environmental Health
Mahidol University
Keywords: Immunology and Microbiology
Issue Date: 1-Mar-2010
Citation: Experimental Parasitology. Vol.124, No.3 (2010), 325-329
Abstract: Recent increasing number of travelers, immigrants and foreign workers from schistosomiasis endemic area has thus resulted in the importation of schistosomiasis to non-endemic countries. To avoid ova-induced pathogenicity, sensitive and specific diagnostic means at an early stage of infection are therefore crucial. In this study, we developed polymerase chain reaction (PCR) primers specific for human schistosome species. The PCR products were obtained in a species-specific manner (479. bp, Schistosoma mansoni; 365. bp, S. haematobium; 614. bp, S. japonicum; 303. bp, S. mekongi) and were detectable from 0.01. pg of total worm DNA (S. haematobium, S. japonicum, S. mekongi). The primer sets were also available for multiplex use. Although some difficulties were experienced in amplifying the parasite DNA from the infected animals, schistosome DNA could be detected from one day post infection. The PCR method described herein will therefore be beneficial to detect human schistosomiasis, after some improvements in this method. © 2009 Elsevier Inc.
ISSN: 10902449
Appears in Collections:Scopus 2006-2010

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