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Title: Multispecies detection of antibodies to influenza A viruses by a double-antigen sandwich ELISA
Authors: Kamolwan Watcharatanyatip
Sirikwan Boonmoh
Kridsada Chaichoun
Taweesak Songserm
Mingkhwan Woratanti
Tararaj Dharakul
Mahidol University
Kasetsart University
Keywords: Immunology and Microbiology
Issue Date: 1-Feb-2010
Citation: Journal of Virological Methods. Vol.163, No.2 (2010), 238-243
Abstract: A double-antigen sandwich ELISA was developed for the detection of antibodies to influenza A viruses. A recombinant nucleoprotein (rNP) of influenza A virus was used as a capture antigen and an HRP-conjugate for detecting the antibodies. A total of 125 serum samples from birds of different species including chickens, geese, open-billed storks, Khaki Campbell ducks, lesser whistling ducks, and pigeons with known antibodies were tested by ELISA. The sensitivity and the specificity of ELISA were found to be 98% and 97.3%, respectively. The assay was able to detect the presence of influenza A antibodies as early as the fourth day post-inoculation in ducks infected experimentally with influenza A (H5N1) virus. Excellent agreement (97.6%) was obtained between this sandwich ELISA and the hemagglutination inhibition (HI) tests (κ = 0.95). The double-antigen sandwich ELISA correlated well with a commercial avian influenza (AI) multispecies ELISA and was slightly more sensitive than the AI multispecies ELISA. These findings indicate that the double-antigen sandwich ELISA based on rNP may offer an effective screening method for serodiagnosis of influenza A virus. The double-antigen sandwich ELISA also enables the detection of antibodies to influenza A viruses in different species without the need for species-specific secondary antibodies. © 2009 Elsevier B.V. All rights reserved.
ISSN: 01660934
Appears in Collections:Scopus 2006-2010

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