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Title: 1,5-Anhydroglucitol attenuates cytokine release and protects mice with type 2 diabetes from inflammatory reactions
Authors: X. Meng
S. Tancharoen
K. I. Kawahara
Y. Nawa
S. Taniguchi
T. Hashiguchi
Ikuro Maruyama
Kagoshima University Faculty of Medicine
Mahidol University
Keywords: Immunology and Microbiology;Medicine;Pharmacology, Toxicology and Pharmaceutics
Issue Date: 1-Jan-2010
Citation: International Journal of Immunopathology and Pharmacology. Vol.23, No.1 (2010), 105-119
Abstract: 1,5-anhydrogIucitol (1,5-AG) decreases in diabetic patients and is used as a marker of glycemic control. Type 2 diabetic patients are susceptibile to lipopolysaccharides (LPS), which stimulate macrophages to release large quantities of tumor necrosis factor (TNF)-α and interleukin (IL)-6. This study examines the effects of 1,5-AG on lung inflammation induced by LPS and consequent systemic inflammation to determine whether the decrease of 1,5-AG concentration induces susceptibility to LPS. Before the challenge with LPS (1 mg/kg in vivo and 500 ng/ml in vitro), we pretreated db/db mice and RAW264.7 cells with 1,5-AG at 38.5 mg/kg and 500 μg/mI, respectively. The levels of IL-6, TNF-α, macrophage chemoattractant protein (MCP)-1 and IL-1β in the serum and in the cell supernatants were measured. We also measured macrophage recruitment and the expression of inducible nitric oxide synthase (iNOS) in pulmonary tissues. We found that 1,5-AG attenuated serum cytokine release and protected db/db mice from LPS-induced pulmonary inflammation. In addition, 1,5-AG suppressed cytokine release and iNOS expression by suppressing Akt/NF-κB activity in RAW264.7 cells. These results suggest that 1,5-AG may be a mediator in, as well as marker for diabetes, and 1,5-AG intake may confer tolerance to LPS in patients with type 2 diabetes. © by Biolife, s.a.s.
ISSN: 03946320
Appears in Collections:Scopus 2006-2010

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