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Title: Development of multiplex polymerase chain reaction for detection of feline hemotropic mycoplasma in blood and tissue specimens
Authors: Parut Suksai
Nareerat Sangkachai
Jarin Chatsiriwech
Oraphan Kanthasaewee
Ladawan Sariya
Kridsada Chaichoun
The Monitoring and Surveillance Center of Zoonotic Diseases in Wildlife and Exotic Animals
Mahidol University
Keywords: Medicine
Issue Date: 1-Nov-2010
Citation: Southeast Asian Journal of Tropical Medicine and Public Health. Vol.41, No.6 (2010), 1447-1453
Abstract: A multiplex polymerase chain reaction (PCR) was developed for the detection of feline hemotropic mycoplasmas which simultaneously differentiates infections of Mycoplasma haemofelis (Mhf), Candidatus Mycoplasma haemominutum (CMhm) and Candidatus Mycoplasma turicensis (CMtc) in feline blood and spleen. These organisms are responsible for the cause of various pathogenicity of feline infectious anemia. These infections are difficult to be detected by microscopic examination, the most commonly used method for general laboratory diagnoses. Specific primers were designed by selected consensus 16S rDNA sequences of three distinct species. The multiplex PCR assay developed in this study was sensitive and specific with detection limit 100 copies/μl DNA of Mhf and CMhm and 10 copies/μl DNA of CMtc. No amplicons could be amplified for other blood parasites or bacterial pathogens. This multiplex PCR will allow studies of pathogenicity and the monitoring of clinical treatment.
ISSN: 01251562
Appears in Collections:Scopus 2006-2010

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