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Title: Copro-DNA diagnosis of Opisthorchis viverrini and Haplorchis taichui infection in an endemic area of LAO PDR
Authors: Megumi Sato
Tiengkham Pongvongsa
Surapol Sanguankiat
Tipparayat Yoonuan
Paron Dekumyoy
Thareerat Kalambaheti
Malaythong Keomoungkhoun
Inthava Phimmayoi
Boungnong Boupha
Kazuhiko Moji
Jitra Waikagul
Mahidol University
Station of Malariology
Institute of Public Health
National Institutes for the Humanities, Research Institute for Humanity and Nature
Keywords: Medicine
Issue Date: 1-Jan-2010
Citation: Southeast Asian Journal of Tropical Medicine and Public Health. Vol.41, No.1 (2010), 28-35
Abstract: The utility of differential copro-DNA diagnosis using modified sample preparation steps of small liver and minute intestinal fluke infections was tested. Fecal samples containing parasite eggs were washed extensively with diluted detergent solution. Parasite eggs were concentrated by sedimentation and broken by microwaving before DNA extraction. PCR targeting ITS1 and ITS2 regions were performed using primer specific for Opisthorchis viverrini, Haplorchis taichui and other related species. Of 125 fecal samples, 94 were positive for small trematode eggs by a modified cellophane thick smear method. By ITS1-PCR, 52 samples were positive for O. viverrini, 12 H. taichui and 7 mixed infection. By ITS2-PCR, 63 were positive for O. viverini, 17 H. taichui, and 19 mixed infection. The ITS-PCR assay identified a higher number of opisthorchiasis cases than those with O. viverrini expelled after treatment, but for H. taichui, ITS-PCR identified less than half of the worm expelled cases. These results showed that copro-DNA diagnosis was useful for the differential diagnosis of O. viverrini and H. taichui infection, which could not be discriminated by microscopy.
ISSN: 01251562
Appears in Collections:Scopus 2006-2010

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