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Title: DNA barcode identification of freshwater snails in the family bithyniidae from Thailand
Authors: Jutharat Kulsantiwong
Sattrachai Prasopdee
Jiraporn Ruangsittichai
Wipaporn Ruangjirachuporn
Thidarut Boonmars
Vithoon Viyanant
Paola Pierossi
Paul D N Hebert
Smarn Tesana
Khon Kaen University
Mahidol University
Thammasat University
University of Guelph
Keywords: Agricultural and Biological Sciences;Biochemistry, Genetics and Molecular Biology;Medicine
Issue Date: 4-Nov-2013
Citation: PLoS ONE. Vol.8, No.11 (2013)
Abstract: Freshwater snails in the family Bithyniidae are the first intermediate host for Southeast Asian liver fluke (Opisthorchis viverrini), the causative agent of opisthorchiasis. Unfortunately, the subtle morphological characters that differentiate species in this group are not easily discerned by non-specialists. This is a serious matter because the identification of bithyniid species is a fundamental prerequisite for better understanding of the epidemiology of this disease. Because DNA barcoding, the analysis of sequence diversity in the 5' region of the mitochondrial COI gene, has shown strong performance in other taxonomic groups, we decided to test its capacity to resolve 10 species/ subspecies of bithyniids from Thailand. Our analysis of 217 specimens indicated that COI sequences delivered species-level identification for 9 of 10 currently recognized species. The mean intraspecific divergence of COI was 2.3% (range 0-9.2%), whereas sequence divergences between congeneric species averaged 8.7% (range 0-22.2%). Although our results indicate that DNA barcoding can differentiate species of these medically-important snails, we also detected evidence for the presence of one overlooked species and one possible case of synonymy. © 2013 Kulsantiwong et al.
ISSN: 19326203
Appears in Collections:Scopus 2011-2015

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