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Title: Sperm quality and the morphology of cryopreserved testicular tissues recovered post-mortem from diverse wild species
Authors: Paweena Thuwanut
Sayamon Srisuwatanasagul
Grisnarong Wongbandue
Nae Tanpradit
Ampika Thongpakdee
Daraka Tongthainan
Sukanya Manee-in
Kaywalee Chatdarong
Chulalongkorn University
Zoological Park Organization, Bangkok
Mahidol University
Keywords: Agricultural and Biological Sciences;Biochemistry, Genetics and Molecular Biology;Medicine
Issue Date: 1-Oct-2013
Citation: Cryobiology. Vol.67, No.2 (2013), 244-247
Abstract: This study compared the effects of slow and fast freezing of testicular tissue of wild animals collected at post-mortem on testicular structure and testicular sperm. The testes of seven animals that had died in captivity; three felids (jungle cat, lion and leopard), two cervids (rusa deer and fea's muntjac) and two bovids (Sumatran serows) were cryopreserved using slow- and fast-freezing protocols. There were greater reductions in the integrity of the sperm membrane and DNA in tissues cryopreserved using slow freezing compared to fast freezing (membrane integrity reduced by 21.5. ±. 12.4% vs. 13.0. ±. 6.9%, P= 0.11 and DNA integrity reduced by 22.7. ±. 16.3% vs. 6.6. ±. 6.3%, P= 0.13). Histologically, there were similar degrees of detachment and shrinkage of the seminiferous tubules whereas, TUNEL assay revealed a tendency towards more apoptotic changes in the intra-tubular cells of tissues frozen using fast freezing compared to slow freezing (P= 0.09). In conclusion, fast freezing tended to cause less damage to testicular sperm but its protective effect on intra-tubular cells was likely compromised. This is the first report of gamete recovery in the wild and of the comparison in various wildlife species, between testicular tissues cryopreserved using different protocols. © 2013 Elsevier Inc.
ISSN: 10902392
Appears in Collections:Scopus 2011-2015

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