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Title: Species-specific primers designed from RAPD products for Bithynia funiculata, the first intermediate host of liver fluke, Opisthorchis viverrini, in north Thailand
Authors: Jutharat Kulsantiwong
Sattrachai Prasopdee
Supawadee Piratae
Panita Khampoosa
Apiporn Suwannatrai
Wipada Duangprompo
Thidarut Boonmars
Wipaporn Ruangjirachuporn
Jiraporn Ruangsittichai
Vithoon Viyanant
Paul D.N. Hebert
Smarn Tesana
Khon Kaen University
Mahidol University
Thammasat University
University of Guelph
Keywords: Agricultural and Biological Sciences;Immunology and Microbiology;Medicine
Issue Date: 1-Jun-2013
Citation: Journal of Parasitology. Vol.99, No.3 (2013), 433-437
Abstract: Bithynia funiculata is the first intermediate host of the human liver fluke Opisthorchis viverrini in northern Thailand but its identification through morphological analysis is often problematic due to the shortage of gastropod taxonomists. As a consequence, we focused on the development of species-specific primers for use as an identification tool. Our work involved recovery of a 502-base pair (bp) amplicon of unknown function through species-specific primers whose effectiveness was tested by analyzing specimens of B. funiculata from 3 locations in northern Thailand. This primer set did not amplify other species in the Bithyniidae or in other gastropod families. By providing a tool to confirm morphological identifications of B. funiculata, and by enabling the identification of juvenile specimens and those with damaged shells, these primers will improve estimates of the prevalence of parasitic infections in this snail. © 2013 American Society of Parasitologists.
ISSN: 19372345
Appears in Collections:Scopus 2011-2015

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