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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/31032
Title: In vitro culture of feline embryos increases stress-induced heat shock protein 70 and apoptotic related genes
Authors: Thanida Sananmuang
Nawapen Phutikanit
Catherine Nguyen
Sukanya Manee-In
Mongkol Techakumphu
Theerawat Tharasanit
Chulalongkorn University
Technologie Avancee pour le Genome et la Clinique
Mahidol University
Keywords: Agricultural and Biological Sciences
Issue Date: 29-Apr-2013
Citation: Journal of Reproduction and Development. Vol.59, No.2 (2013), 180-188
Abstract: Developmental competence and quality of in vitro produced embryos has been demonstrated to be lower than in vivo derived embryos. This study aimed specifically to determine the effects of in vitro culture of feline embryos using various culture densities on developmental competence and expression of stress- and apoptotic-related genes in terms of heat shock protein 70 (HSP70) and apoptotic-related (BAX and BCL-2) gene expressions. In experiment 1, we characterized the inducible form of a feline-specific HSP70 mRNA sequence, as it has not been previously reported. The primers for feline HSP70 mRNA were synthesized and tested on heat-treated cat fibroblasts. In experiment 2, feline embryos were cultured at different culture densities (embryo: culture volume; 1:1.25, 1:5 and 1:20). The developmental competence was determined along with HSP70, BAX and BCL-2 transcript abundances using quantitative RT-PCR. In vivo derived embryos were used as a control group. A partial cat HSP70 mRNA sequence (190 bp) was characterized and exhibited high nucleotide identity (93 to 96%) with other species. Cleaved embryos cultured at high density (1:1.25) developed to blastocysts at a lower rate than those generated from lower densities. Irrespective of the culture densities used, in vitro cultured blastocysts showed increased levels of HSP70 and BAX transcripts compared with in vivo counterparts. Blastocysts derived from the highest culture density (1:1.25) showed higher levels of upregulation of HSP70 and BAX transcripts than those cultured at lower culture densities (1:5 and 1:20). In conclusion, increased levels of pro-apoptotic (BAX) and stress-response (HSP70) transcripts correlated with developmental incompetence of embryos cultured at high embryonic density, indicating that stress accumulated during in vitro embryo culture affected the fate for embryo development and quality. © 2013 by the Society for Reproduction and Development.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84876516593&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/31032
ISSN: 13484400
09168818
Appears in Collections:Scopus 2011-2015

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