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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/31048
Title: Evaluation of In Vitro Cross-Reactivity to Avian H5N1 and Pandemic H1N1 2009 Influenza Following Prime Boost Regimens of Seasonal Influenza Vaccination in Healthy Human Subjects: A Randomised Trial
Authors: Delia Bethell
David Saunders
Anan Jongkaewwattana
Jarin Kramyu
Arunee Thitithayanont
Suwimon Wiboon-ut
Kosol Yongvanitchit
Amporn Limsalakpetch
Utaiwan Kum-Arb
Nichapat Uthaimongkol
Jean Michel Garcia
Ans E. Timmermans
Malik Peiris
Stephen Thomas
Anneke Engering
Richard G. Jarman
Duangrat Mongkolsirichaikul
Carl Mason
Nuanpan Khemnu
Stuart D. Tyner
Mark M. Fukuda
Douglas S. Walsh
Sathit Pichyangkul
Armed Forces Research Institute of Medical Sciences, Thailand
Thailand National Center for Genetic Engineering and Biotechnology
Mahidol University
The University of Hong Kong
Keywords: Agricultural and Biological Sciences;Biochemistry, Genetics and Molecular Biology
Issue Date: 26-Mar-2013
Citation: PLoS ONE. Vol.8, No.3 (2013)
Abstract: Introduction: Recent studies have demonstrated that inactivated seasonal influenza vaccines (IIV) may elicit production of heterosubtypic antibodies, which can neutralize avian H5N1 virus in a small proportion of subjects. We hypothesized that prime boost regimens of live and inactivated trivalent seasonal influenza vaccines (LAIV and IIV) would enhance production of heterosubtypic immunity and provide evidence of cross-protection against other influenza viruses. Methods: In an open-label study, 26 adult volunteers were randomized to receive one of four vaccine regimens containing two doses of 2009-10 seasonal influenza vaccines administered 8 (±1) weeks apart: 2 doses of LAIV; 2 doses of IIV; LAIV then IIV; IIV then LAIV. Humoral immunity assays for avian H5N1, 2009 pandemic H1N1 (pH1N1), and seasonal vaccine strains were performed on blood collected pre-vaccine and 2 and 4 weeks later. The percentage of cytokine-producing T-cells was compared with baseline 14 days after each dose. Results: Subjects receiving IIV had prompt serological responses to vaccine strains. Two subjects receiving heterologous prime boost regimens had enhanced haemagglutination inhibition (HI) and neutralization (NT) titres against pH1N1, and one subject against avian H5N1; all three had pre-existing cross-reactive antibodies detected at baseline. Significantly elevated titres to H5N1 and pH1N1 by neuraminidase inhibition (NI) assay were observed following LAIV-IIV administration. Both vaccines elicited cross-reactive CD4+ T-cell responses to nucleoprotein of avian H5N1 and pH1N1. All regimens were safe and well tolerated. Conclusion: Neither homologous nor heterologous prime boost immunization enhanced serum HI and NT titres to 2009 pH1N1 or avian H5N1 compared to single dose vaccine. However heterologous prime-boost vaccination did lead to in vitro evidence of cross-reactivity by NI; the significance of this finding is unclear. These data support the strategy of administering single dose trivalent seasonal influenza vaccine at the outset of an influenza pandemic while a specific vaccine is being developed. Trial Registration: ClinicalTrials.gov NCT01044095. © 2013 Bethell, et al.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84875448994&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/31048
ISSN: 19326203
Appears in Collections:Scopus 2011-2015

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