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dc.contributor.authorP. Imraten_US
dc.contributor.authorP. Suthanmapinanthen_US
dc.contributor.authorK. Saikhunen_US
dc.contributor.authorS. Mahasawangkulen_US
dc.contributor.authorE. Sostaricen_US
dc.contributor.authorP. Sombutputornen_US
dc.contributor.authorS. Jansittiwateen_US
dc.contributor.authorN. Thongtipen_US
dc.contributor.authorA. Pinyopumminen_US
dc.contributor.authorB. Colenbranderen_US
dc.contributor.authorW. V. Holten_US
dc.contributor.authorT. A.E. Stouten_US
dc.contributor.otherUtrecht Universityen_US
dc.contributor.otherKasetsart Universityen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherThailand Forest Industry Organizationen_US
dc.contributor.otherZoological Society of London Institute of Zoologyen_US
dc.contributor.otherUniversiteit van Pretoriaen_US
dc.identifier.citationCryobiology. Vol.66, No.1 (2013), 52-59en_US
dc.description.abstractSemen cryopreservation and artificial insemination (AI) are potentially valuable methods for supporting the breeding management of endangered species like the Asian elephant. Cryopreservation of Asian elephant semen has however proven problematic with respect to maintenance of both adequate semen quality and fertility post-thaw. In this study, nine ejaculates from three adult bulls were used to compare the influence of extender (TEST versus INRA96®) and penetrating cryoprotectants (3% glycerol, 5% glycerol and 4% methylformamide) on post-thaw semen quality. We demonstrate that not only the freezing process, but also the quality of the semen before freezing, significantly influences the freezability of Asian elephant semen. Pre-freeze motility, viability, semen volume, semen pH, sperm concentration and the incidence of sperm mid-piece and tail abnormalities all significantly (. p<. 0.05) affected post-thaw semen quality. While extender and cryoprotectant did not significantly affect any of the above semen quality parameters post-thaw, the skim-milk based extender (INRA96®) preserved DNA integrity better (. p<. 0.05) than the egg yolk extender (TEST). Considerable between-ejaculate variation in all post-thaw semen quality parameters was also noted. It is concluded that strict criteria for semen quality is essential for the selection of Asian elephant bull ejaculates suitable for cryopreservation; stricter initial selection should improve the mean post-thaw quality. © 2012 Elsevier Inc.en_US
dc.rightsMahidol Universityen_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleEffect of pre-freeze semen quality, extender and cryoprotectant on the post-thaw quality of Asian elephant (Elephas maximus indicus) semenen_US
Appears in Collections:Scopus 2011-2015

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