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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/31176
Title: Base excision DNA repair defect in thioredoxin-1 (Trx1)-deficient cells
Authors: Hye Lim Kim
Preeyaporn Koedrith
Sang Min Lee
Yeo Jin Kim
Young Rok Seo
Dongguk University, Seoul
Faculty of Environment and Resource Studies, Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology;Environmental Science
Issue Date: 1-Nov-2013
Citation: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis. Vol.751-752, No.1 (2013), 1-7
Abstract: Thioredoxin-1 (Trx1) is an antioxidant enzyme with a protective role in the removal of oxidative stress. We investigated the mechanism by which the redox modulator Trx1 affects base excision repair (BER) activity to understand the protective role of Trx1. We constructed a Trx1 knockdown system to demonstrate the specific mechanism of Trx1. DNA damage in terms of relative intensity of the DNA tail and γ-H2AX foci was markedly higher in the Trx1 shRNA cells compared with that in the wild type cells, leading to increased cellular susceptibility to a sublethal dose of BER-inducible toxicant, nitrosomethylurea (NMU). In addition, we observed a modulatory role of Trx1 in the BER pathway via the p53 downstream gene, growth arrest, and DNA-damage-inducible protein 45 α (Gadd45a). The protein level and function of p53, a Trx1 downstream gene, coincidently decreased in the Trx1 shRNA cells. Furthermore, Trx1 shRNA cells showed decreased Gadd45a expression and interaction of Gadd45a with apurinic/apyrimidinic endonuclease 1 (APE1) as well as APE1 activity. In conclusion, Trx1 might cooperate in the control of APE1 function by modulating the p53-mediated BER via the protein-protein interaction between Gadd45a and APE1, providing insight into the novel role of redox factor Trx1 in modulation of BER. © 2013 Elsevier B.V.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84888057720&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/31176
ISSN: 09218262
00275107
Appears in Collections:Scopus 2011-2015

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