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Title: ABO blood-typing using an antibody array technique based on surface plasmon resonance imaging
Authors: Nongluck Houngkamhang
Apirom Vongsakulyanon
Patjaree Peungthum
Krisda Sudprasert
Pimpun Kitpoka
Mongkol Kunakorn
Boonsong Sutapun
Ratthasart Amarit
Armote Somboonkaew
Toemsak Srikhirin
Mahidol University
Suranaree University of Technology
Thailand National Electronics and Computer Technology Center
Keywords: Biochemistry, Genetics and Molecular Biology;Chemistry;Engineering;Physics and Astronomy
Issue Date: 9-Sep-2013
Citation: Sensors (Switzerland). Vol.13, No.9 (2013), 11913-11922
Abstract: In this study, readily available antibodies that are used in standard agglutination tests were evaluated for their use in ABO blood typing by a surface plasmon resonance imaging (SPR imaging) technique. Five groups of antibodies, including mixed clones of anti-A, anti-B, and anti-AB, and single clones of anti-A and anti-B, were used to construct the five-line detection arrays using a multichannel flow cell in the SPR imager. The red blood cell (RBC) samples were applied to a multichannel flow cell that was orthogonal to the detection line arrays for blood group typing. We found that the blood samples were correctly grouped in less than 12 min by the SPR imaging technique, and the results were consistent with those of the standard agglutination technique for all 60 samples. We found that mixed clones of antibodies provided 33%-68% greater change in the SPR signal than the single-clone antibodies. Applying the SPR imaging technique using readily available antibodies may reduce the costs of the antibodies, shorten the measurement time, and increase the throughput. © 2013 by the authors; licensee MDPI, Basel, Switzerland.
ISSN: 14248220
Appears in Collections:Scopus 2011-2015

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