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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/31253
Title: Serotype-specific anti-dengue virus NS1 mouse antibodies cross-react with prM and are potentially involved in virus production
Authors: Promsin Masrinoul
Magot Diata Omokoko
Sabar Pambudi
Kazuyoshi Ikuta
Takeshi Kurosu
Osaka University
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology;Immunology and Microbiology
Issue Date: 1-Aug-2013
Citation: Viral Immunology. Vol.26, No.4 (2013), 250-258
Abstract: Dengue virus (DENV) infection induces a strong B-cell immune response against the viral nonstructural protein 1 (NS1). Anti-NS1 antibodies (Abs) may affect virus production because they coexist with the virus in the patients' blood. The present study examined whether ten mouse monoclonal antibodies (MAbs) raised against NS1 affected production of the DENV-2. Three MAbs, 4C2, 4G11, and 4E5, showed weak neutralizing activity in a focus reduction assay. In addition, two serotype-specific MAbs, 4C2 and 4G11, protected suckling mice from lethal infection with DENV-2. An immunoprecipitation assay with DENV-2 showed that these MAbs, which were specific for the NS1 of DENV-4 and DENV-1, cross-reacted with the DENV-2 pre-membrane (prM) protein, but not with DENV-2 NS1. Interestingly, high concentrations of MAb 4G11 showed antibody-dependent enhancement of DENV-2 infection in human monocyte THP-1 cells. Taken together, these observations suggest that serotype-specific anti-NS1 MAbs are potentially involved in virus production. © 2013, Mary Ann Liebert, Inc.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84882400783&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/31253
ISSN: 15578976
08828245
Appears in Collections:Scopus 2011-2015

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