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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/31304
Title: Sustained replication of dengue pseudoinfectious virus lacking the capsid gene by trans-complementation in capsid-producing mosquito cells
Authors: Sutha Sangiambut
Amporn Suphatrakul
Rungtawan Sriburi
Poonsook Keelapang
Chunya Puttikhunt
Watchara Kasinrerk
Prida Malasit
Nopporn Sittisombut
Thailand National Center for Genetic Engineering and Biotechnology
Chiang Mai University
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology;Immunology and Microbiology;Medicine
Issue Date: 1-Jun-2013
Citation: Virus Research. Vol.174, No.1-2 (2013), 37-46
Abstract: A simple system for the generation of pseudoinfectious particles of dengue virus was developed to facilitate studies of virus replication and vaccine development. Selected clones of the C6/36 mosquito cell line expressing an anchored form of the dengue virus capsid protein served as host cells for the trans-complementation of partially capsid-deleted viral RNA generated in vitro. Transfection of the partially capsid-deleted viral RNA into the anchored capsid-expressing C6/36 cells resulted in moderate titers of infectious virus. Progeny viruses multiplied in the capsid trans-complementing C6/36 cells for up to three weeks, but only initiated single rounds of replication in Vero cells lacking the capsid protein. Employing this trans-complementation system, it was found that nearly all of the capsid-coding sequence in the viral RNA was dispensable for the generation of pseudoinfectious dengue virus particles in mosquito cells. © 2013 Elsevier B.V.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84877624305&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/31304
ISSN: 18727492
01681702
Appears in Collections:Scopus 2011-2015

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