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Title: The hdhA gene encodes a haloacid dehalogenase that is regulated by the Lysr-type regulator, HdhR, in sinorhizobium meliloti
Authors: Ratiboot Sallabhan
Jarunee Kerdwong
James M. Dubbs
Kumpanart Somsongkul
Wirongrong Whangsuk
Phairin Piewtongon
Skorn Mongkolsuk
Suvit Loprasert
Chulabhorn Research Institute
Mahidol University
South Carolina Commission on Higher Education
Chulabhorn Graduate Institute
Keywords: Biochemistry, Genetics and Molecular Biology;Chemical Engineering;Immunology and Microbiology
Issue Date: 1-Jun-2013
Citation: Molecular Biotechnology. Vol.54, No.2 (2013), 148-157
Abstract: The plasmid pSymA, in the nitrogen-fixing soil bacterium, Sinorhizobium meliloti, carries a 750-bp ORF (SMa1978) designated, hdhA, which encodes a novel dehalogenase that can detoxify haloacid compounds, showing a preference for haloacetic acids. Purified His-tagged HdhA demonstrated the apparent ability to dehalogenate chloroacetic acid and trifluoroacetic acid. In addition, upstream of hdhA, a gene encoding a lysR-type transcription regulator denoted, hdhR (SMa1979), has been identified to be a transcriptional repressor of hdhA expression. In an hdhR knockout mutant, hdhA promoter activity was markedly increased. Purified 32-kDa His-tagged HdhR repressed expression of hdhA by specifically binding to the promoter region of hdhA, as demonstrated by gel mobility shift assay and DNase I foot printing experiments. Moreover, the pesticide, pentachlorophenol, was also found to induce hdhA expression via HdhR. Site-directed mutants, in which the Cys residues at positions 160 and 192 in HdhR were changed to Ser, were constructed. C160S and C192S single mutants showed diminished HdhR-mediated repression of hdhA expression, while a C160S:C192S double mutant could no longer repress expression of hdhA. © 2012 Springer Science+Business Media, LLC.
ISSN: 15590305
Appears in Collections:Scopus 2011-2015

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