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dc.contributor.authorNantana Nuchtavornen_US
dc.contributor.authorPetr Smejkalen_US
dc.contributor.authorMichael C. Breadmoreen_US
dc.contributor.authorRosanne M. Guijten_US
dc.contributor.authorPhilip Dobleen_US
dc.contributor.authorFritz Beken_US
dc.contributor.authorFrantisek Foreten_US
dc.contributor.authorLeena Suntornsuken_US
dc.contributor.authorMirek Mackaen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherDublin City Universityen_US
dc.contributor.otherInstitute of Analytical Chemistry of the Czech Academy of Sciencesen_US
dc.contributor.otherUniversity of Tasmaniaen_US
dc.contributor.otherUniversity of Technology Sydneyen_US
dc.contributor.otherAgilent Technologies Deutschland GmbHen_US
dc.identifier.citationJournal of Chromatography A. Vol.1286, (2013), 216-221en_US
dc.description.abstractMicrofluidic chip electrophoresis (chip-CE) is a separation method that is compatible with portable and on-site analysis, however, only few commercial chip-CE systems with laser-induced fluorescence (LIF) and light emitting diode (LED) fluorescence detection are available. They are established for several application tailored methods limited to specific biopolymers (DNA, RNA and proteins), and correspondingly the range of their applications has been limited. In this work we address the lack of commercially available research-type flexible chip-CE platforms by exploring the limits of using an application-tailored system equipped with chips and methods designed for DNA separations as a generic chip-CE platform - this is a very significant issue that has not been widely studied. In the investigated Agilent Bioanalyzer chip-CE system, the fixed components are the Agilent chips and the detection (LIF at 635nm and LEDIF at 470nm), while the chemistry (electrolyte) and the programming of all the high voltages are flexible. Using standard DNA chips, we show that a generic CE function of the system is easily possible and we demonstrate an extension of the applicability to non-aqueous CE (NACE). We studied the chip compatibility with organic solvents (i.e. MeOH, ACN, DMF and DMSO) and demonstrated the chip compatibility with DMSO as a non-volatile and non-hazardous solvent with satisfactory stability of migration times over 50h. The generic CE capability is illustrated with separations of fluorescent basic blue dyes methylene blue (MB), toluidine blue (TB), nile blue (NB) and brilliant cresyl blue (BC). Further, the effects of the composition of the background electrolyte (BGE) on the separation were studied, including the contents of water (0-30%) and buffer composition. In background electrolytes containing typically 80mmol/L ammonium acetate and 870mmol/L acetic acid in 100% DMSO baseline separation of the dyes were achieved in 40s. Linearity was documented in the range of 5-28μmol/L, 10-100μmol/L, 1.56-50nmol/L and 5-75nmol/L (r2values in the range 0.974-0.999), and limit of detection (LOD) values were 90nmol/L, 1μmol/L 1.4nmol/L, and 2nmol/L for MB, TB, NB and BC, respectively. © 2013 Elsevier B.V.en_US
dc.rightsMahidol Universityen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleExploring chip-capillary electrophoresis-laser-induced fluorescence field-deployable platform flexibility: Separations of fluorescent dyes by chip-based non-aqueous capillary electrophoresisen_US
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