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Title: Modification of human cancellous bone using Thai silk fibroin and gelatin for enhanced osteoconductive potential
Authors: Rungnapa Vorrapakdee
Sorada Kanokpanont
Juthamas Ratanavaraporn
Saranatra Waikakul
Chris Charoenlap
Siriporn Damrongsakkul
Chulalongkorn University
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology;Chemical Engineering;Engineering;Materials Science
Issue Date: 1-Mar-2013
Citation: Journal of Materials Science: Materials in Medicine. Vol.24, No.3 (2013), 735-744
Abstract: The modification of human cancellous bone (hBONE) with silk fibroin/gelatin (SF/G) using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC)/N-hydroxysuccini-mide (NHS) crosslinking was established. The SF/G solutions at a weight ratio of 50/50 and the solution concentrations of 1, 2, and 4 wt % were studied. SF/G sub-matrix was formed on the surface and inside pore structure of hBONE. All hBONE scaffolds modified with SF/G showed smaller pore sizes, less porosity, and slightly lower compressive modulus than unmodified hBONE. SF/G sub-matrix was gradually biodegraded in collagenase solution along 4 days. The hBONE scaffolds modified with SF/G, particularly at 2 and 4 wt % solution concentrations, promoted attachment, proliferation, and osteogenic differentiation of bone marrow-derived mesenchymal stem cells (MSC), comparing to the original hBONE. The highest cell number, ALP activity and calcium production were observed for MSC cultured on the hBONE scaffolds modified with 4 wt % SF/G. The mineralization was also remarkably induced in the cases of modified hBONE scaffolds as observed from the deposited calcium phosphate by EDS. The modification of hBONE with SF/G was, therefore, the promising method to enhance the osteoconductive potential of human bone graft for bone tissue engineering. © 2012 Springer Science+Business Media New York.
ISSN: 15734838
Appears in Collections:Scopus 2011-2015

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