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Title: Sensitivity and specificity of PS/AA-modified nanoparticles used in malaria detection
Authors: Raweewan Thiramanas
Kulachart Jangpatarapongsa
Udom Asawapirom
Pramuan Tangboriboonrat
Duangporn Polpanich
Thailand National Science and Technology Development Agency
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology;Chemical Engineering;Immunology and Microbiology
Issue Date: 1-Jan-2013
Citation: Microbial Biotechnology. Vol.6, No.4 (2013), 406-413
Abstract: Polystyrene (PS) nanoparticle (NP) copolymerized with acrylic acid (AA) and coloured monomer, i.e. 2,3,6,7-tetra(2,2′-bithiophene)-1,4,5,8-naphthalenetetracarboxylic-N,N′-di(2-methylallyl)-bisimide (ALN8T), was synthesized via the miniemulsion polymerization. Before applying for malaria antigen detection, the blue NP was conjugated with human polyclonal malaria IgG antibody (Ab) specific to Plasmodium falciparum. For the conjugation, three methods, i.e. physical adsorption, covalent coupling and affinity binding via streptavidin (SA) and biotin interaction, were employed. The optimum ratio of Ab to NPs used in each immobilization procedure and the latex agglutination test based on the reaction between Ab conjugated NPs and malaria patient plasma were investigated. All Ab-latex conjugates provided the high sensitivity for the detection of P.falciparum malaria plasma. The highest specificity to P.falciparum was obtained from using Ab-NPs conjugated via the SA-biotin interaction. © 2013 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.
ISSN: 17517915
Appears in Collections:Scopus 2011-2015

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