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dc.contributor.authorKamphon Intharanuten_US
dc.contributor.authorRudi Gramsen_US
dc.contributor.authorSasitorn Bejrachandraen_US
dc.contributor.authorPramote Sriwanitchraken_US
dc.contributor.authorOytip Nathalangen_US
dc.contributor.otherThammasat Universityen_US
dc.contributor.otherMahidol Universityen_US
dc.identifier.citationJournal of Clinical Laboratory Analysis. Vol.27, No.1 (2013), 53-58en_US
dc.description.abstractBackground: We developed an allele-specific polymerase chain reaction (AS-PCR) technique for Kidd blood group genotyping. Methods: Altogether, 340 blood samples from Thai blood donors at the National Blood Centre, Thai Red Cross Society, were tested with anti-Jka and anti-Jkb using the gel technique and the direct urea lysis test was used for screening Jk(a-b-) phenotype. For AS-PCR technique, different types of primers were used for JK*01 and JK*02 allele detections in known DNA controls. Results: Regarding JK*02 allele detection, the pseudopositve amplification products were found when using correctly matched forward primer and a single mismatch forward primer. Interestingly, one type of two mismatch pairing at the 3′ end of the forward primer can be used together with the newly designed reverse primer for Kidd blood group genotyping. It was found that the typing results in all samples obtained by serological techniques and newly developed AS-PCR technique were in agreement and this PCR technique also gave 100% concordance of results in 30 samples randomly tested twice and demonstrated reproducible results. Conclusion: This study shows that the in-house AS-PCR is simple, cost-effective, and convenient for Kidd blood group genotyping in routine laboratories, especially, in resolving serologic investigations. © 2012 Wiley Periodicals, Inc.en_US
dc.rightsMahidol Universityen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectHealth Professionsen_US
dc.titleImproved Allele-Specific PCR Technique for Kidd Blood Group Genotypingen_US
Appears in Collections:Scopus 2011-2015

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