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Title: Detection of vibrio cholerae using the intrinsic catalytic activity of a magnetic polymeric nanoparticle
Authors: Raweewan Thiramanas
Kulachart Jangpatarapongsa
Pramuan Tangboriboonrat
Duangporn Polpanich
Thailand National Science and Technology Development Agency
Mahidol University
Keywords: Chemistry
Issue Date: 18-Jun-2013
Citation: Analytical Chemistry. Vol.85, No.12 (2013), 5996-6002
Abstract: A novel and sensitive magnetic polymeric nanoparticle (MPNP)-polymerase chain reaction-colorimetry (magneto-PCR-colorimetry) technique was developed for detection of Vibrio cholerae (V. cholerae). The technique involved an amplification of V. cholerae DNA on the surface of an MPNP and then employed the intrinsic catalytic activity of the MPNP to detect the target gene by colorimetry. An amino-modified forward primer was covalently labeled onto the MPNP surface which would bind to PCR product during PCR cycling. By employing the catalytic activity of the MPNP, the analysis of PCR product bound MPNP yielded a sensitivity of 103 CFU/mL of V. cholerae in buffer system within 4 h. The specificity and efficiency of the technique were investigated by using various bacterial DNAs in drinking and tap water. © 2013 American Chemical Society.
ISSN: 15206882
Appears in Collections:Scopus 2011-2015

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