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Title: Modulation of antibody responses against Gnathostoma spinigerum in mice immunized with crude antigen formulated in CpG oligonucleotide and montanide ISA720
Authors: Pewpan M. Intapan
Chakrit Hirunpetcharat
Churairat Kularbkaew
Wiboonchai Yutanawiboonchai
Penchom Janwan
Wanchai Maleewong
Khon Kaen University
Mahidol University
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Dec-2013
Citation: Korean Journal of Parasitology. Vol.51, No.6 (2013), 637-644
Abstract: This study aimed to investigate the antibody responses in mice immunized with Gnathostoma spinigerum crude antigen (GsAg) incorporated with the combined adjuvant, a synthetic oligonucleotide containing unmethylated CpG motif (CpG ODN 1826) and a stable water in oil emulsion (Montanide ISA720). Mice immunized with GsAg and combined adjuvant produced all antibody classes and subclasses to GsAg except IgA. IgG2a/2b/3 but not IgG1 subclasses were enhanced by immunization with CpG ODN 1826 when compared with the control groups immunized with non-CpG ODN and Montanide ISA or only with Montanide ISA, suggesting a biased induction of a Th1-type response by CpG ODN. After challenge infection with live G. spinigerum larvae, the levels of IgG2a/2b/3 antibody subclasses decreased immediately and continuously, while the IgG1 subclass remained at high levels. This also corresponded to a continuous decrease of the IgG2a/IgG1 ratio after infection. Only IgM and IgG1 antibodies, but not IgG2a/2b/3, were significantly produced in adjuvant control groups after infection. These findings suggest that G. spinigerum infection potently induces a Th2-type biased response. © 2013, Korean Society for Parasitology and Tropical Medicine.
ISSN: 17380006
Appears in Collections:Scopus 2011-2015

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