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Title: Molecular differentiation of Schistosoma japonicum and Schistosoma mekongi by real-time PCR with high resolution melting analysis
Authors: Amornmas Kongklieng
Worasak Kaewkong
Pewpan M. Intapan
Oranuch Sanpool
Penchom Janwan
Tongjit Thanchomnang
Viraphong Lulitanond
Pusadee Sri-Aroon
Yanin Limpanont
Wanchai Maleewong
Khon Kaen University
Naresuan University
Mahasarakham University
Mahidol University
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Dec-2013
Citation: Korean Journal of Parasitology. Vol.51, No.6 (2013), 651-656
Abstract: Human schistosomiasis caused by Schistosoma japonicum and Schistosoma mekongi is a chronic and debilitating helminthic disease still prevalent in several countries of Asia. Due to morphological similarities of cercariae and eggs of these 2 species, microscopic differentiation is difficult. High resolution melting (HRM) real-time PCR is developed as an alternative tool for the detection and differentiation of these 2 species. A primer pair was designed for targeting the 18S ribosomal RNA gene to generate PCR products of 156 base pairs for both species. The melting points of S. japonicum and S. mekongi PCR products were 84.5±0.07 °C and 85.7±0.07 °C, respectively. The method permits amplification from a single cercaria or an egg. The HRM real-time PCR is a rapid and simple tool for differentiation of S. japonicum and S. mekongi in the intermediate and final hosts. © 2013, Korean Society for Parasitology and Tropical Medicine.
ISSN: 17380006
Appears in Collections:Scopus 2011-2015

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