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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/31845
Title: Short report: Rapid detection of Burkholderia pseudomallei in blood cultures using a monoclonal antibody-based immunofluorescent assay
Authors: Narisara Chantratita
Sarunporn Tandhavanant
Gumphol Wongsuvan
Vanaporn Wuthiekanun
Nittaya Teerawattanasook
Nicholas P.J. Day
Direk Limmathurotsakul
Sharon J. Peacock
Mahidol University
Sappasitthiprasong Hospital
Nuffield Department of Clinical Medicine
University of Cambridge
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Nov-2013
Citation: American Journal of Tropical Medicine and Hygiene. Vol.89, No.5 (2013), 971-972
Abstract: Melioidosis is a severe bacterial infection caused by Burkholderia pseudomallei. Rapid antimicrobial therapy is necessary to improve patient outcome, which is aided by direct detection of B. pseudomallei in clinical samples. A drawback for all antigen assays is that the number of B. pseudomallei in blood usually falls below the achievable level of detection. We performed a prospective cohort study of 461 patients with 541 blood cultures to evaluate the utility of a pre-incubation step prior to detection of B. pseudomallei using a monoclonal antibody-based immunofluorescent assay (Mab-IFA). TheMab-IFA was positive in 74 of 76 patients with melioidosis (sensitivity = 97.4%), and negative in 385 patients who did not have blood cultures containing B. pseudomallei (specificity = 100%). The Mab-IFA could be a valuable supplementary tool for rapid detection. We recommend the use of the Mab-IFA to test blood cultures that flag positive in regions where melioidosis is endemic. Copyright © 2013 by The American Society of Tropical Medicine and Hygiene.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84888604643&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/31845
ISSN: 00029637
Appears in Collections:Scopus 2011-2015

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