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|Title:||Short report: Rapid detection of Burkholderia pseudomallei in blood cultures using a monoclonal antibody-based immunofluorescent assay|
Nicholas P.J. Day
Sharon J. Peacock
Nuffield Department of Clinical Medicine
University of Cambridge
|Keywords:||Immunology and Microbiology;Medicine|
|Citation:||American Journal of Tropical Medicine and Hygiene. Vol.89, No.5 (2013), 971-972|
|Abstract:||Melioidosis is a severe bacterial infection caused by Burkholderia pseudomallei. Rapid antimicrobial therapy is necessary to improve patient outcome, which is aided by direct detection of B. pseudomallei in clinical samples. A drawback for all antigen assays is that the number of B. pseudomallei in blood usually falls below the achievable level of detection. We performed a prospective cohort study of 461 patients with 541 blood cultures to evaluate the utility of a pre-incubation step prior to detection of B. pseudomallei using a monoclonal antibody-based immunofluorescent assay (Mab-IFA). TheMab-IFA was positive in 74 of 76 patients with melioidosis (sensitivity = 97.4%), and negative in 385 patients who did not have blood cultures containing B. pseudomallei (specificity = 100%). The Mab-IFA could be a valuable supplementary tool for rapid detection. We recommend the use of the Mab-IFA to test blood cultures that flag positive in regions where melioidosis is endemic. Copyright © 2013 by The American Society of Tropical Medicine and Hygiene.|
|Appears in Collections:||Scopus 2011-2015|
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