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Title: Therapeutic effect of Artemia enriched with Escherichia coli expressing double-stranded RNA in the black tiger shrimp Penaeus monodon
Authors: Thitiporn Thammasorn
Parinyachat Somchai
Chaowanee Laosutthipong
Sarocha Jitrakorn
Somjai Wongtripop
Siripong Thitamadee
Boonsirm Withyachumnarnkul
Vanvimon Saksmerprome
Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp)
Mahidol University
Thailand National Center for Genetic Engineering and Biotechnology
Shrimp Genetics Improvement Center (SGIC)
Prince of Songkla University
Keywords: Immunology and Microbiology;Pharmacology, Toxicology and Pharmaceutics
Issue Date: 5-Sep-2013
Citation: Antiviral Research. Vol.100, No.1 (2013), 202-206
Abstract: We exploited Artemia as a double-stranded (ds)RNA-delivery system to combat viral diseases in shrimp. First, the transformed Escherichia coli (E. coli) expressing red fluorescent protein (RFP) was tested in the Artemia enrichment process. RFP signals detectable in the gut of Artemia under confocal microscope were evident for the successful encapsulation. Second, the Artemia enrichment process was performed using E. coli producing Laem-Singh virus (LSNV)-specific dsRNA, which has been previously shown to inhibit the viral infection in the black tiger shrimp Penaeus monodon by intramuscular injection and oral administration. The enriched Artemia nauplii were confirmed to contain dsRNA-LSNV by RT-PCR, and were subjected to the feeding test with P. monodon postlarvae. Quantitative RT-PCR indicated that a number of LSNV copies in most of the treated shrimp were, at least, 1000-fold lower than the untreated controls. During 11-17 weeks after feeding, average body weight of the treated group was markedly increased relative to the control group. A smaller differential growth rate of the treated group as compared to the control was also noticed. These results suggested that feeding shrimp with the dsRNA-enriched Artemia can eliminate LSNV infection, which is the cause of retarded growth in P. monodon. The present study reveals for the first time the therapeutic effect of dsRNA-enriched Artemia for shrimp disease control. © 2013 Elsevier B.V. All rights reserved.
ISSN: 18729096
Appears in Collections:Scopus 2011-2015

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