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|Title:||Short report: A prospective evaluation of real-time PCR assays for the detection of Orientia tsutsugamushi and Rickettsia spp. for early diagnosis of rickettsial infections during the acute phase of undifferentiated febrile illness|
Allen L. Richards
Kevin M. Bourzac
Stuart D. Blacksell
Shoklo Malaria Research Unit
University of Oxford
Naval Medical Research Center
BioFire Diagnostics, LLC
|Keywords:||Immunology and Microbiology;Medicine|
|Citation:||American Journal of Tropical Medicine and Hygiene. Vol.89, No.2 (2013), 308-310|
|Abstract:||One hundred and eighty febrile patients were analyzed in a prospective evaluation of Orientia tsutsugamushi and Rickettsia spp. real-time polymerase chain reaction (PCR) assays for early diagnosis of rickettsial infections. By paired serology, 3.9% (7 of 180) and 6.1% (11 of 180) of patients were confirmed to have acute scrub or murine typhus, respectively. The PCR assays for the detection of O. tsutsugamushi and Rickettsia spp. had high specificity (99.4% [95% confidence interval (CI): 96.8-100] and 100%[95%CI: 97.8-100], respectively). The PCR results were also compared with immunoglobulinM(IgM) immunofluorescence assay (IFA) on acute sera. For O. tsutsugamushi, PCR sensitivity was twice that of acute specimen IgM IFA (28.6% versus 14.3%; McNemar's P = 0.3). For Rickettsia spp., PCR was four times as sensitive as acute specimen IgM IFA (36.4% versus 9.1%; P = 0.08), although this was not statistically significant. Whole blood and buffy coat, but not serum, were acceptable specimens for these PCRs. Further evaluation of these assays in a larger prospective study is warranted. Copyright © 2013 by The American Society of Tropical Medicine and Hygiene.|
|Appears in Collections:||Scopus 2011-2015|
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