Simple jQuery Dropdowns
Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/31894
Title: Short report: A prospective evaluation of real-time PCR assays for the detection of Orientia tsutsugamushi and Rickettsia spp. for early diagnosis of rickettsial infections during the acute phase of undifferentiated febrile illness
Authors: Wanitda Watthanaworawit
Paul Turner
Claudia Turner
Ampai Tanganuchitcharnchai
Allen L. Richards
Kevin M. Bourzac
Stuart D. Blacksell
François Nosten
Shoklo Malaria Research Unit
Mahidol University
University of Oxford
Naval Medical Research Center
BioFire Diagnostics, LLC
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Aug-2013
Citation: American Journal of Tropical Medicine and Hygiene. Vol.89, No.2 (2013), 308-310
Abstract: One hundred and eighty febrile patients were analyzed in a prospective evaluation of Orientia tsutsugamushi and Rickettsia spp. real-time polymerase chain reaction (PCR) assays for early diagnosis of rickettsial infections. By paired serology, 3.9% (7 of 180) and 6.1% (11 of 180) of patients were confirmed to have acute scrub or murine typhus, respectively. The PCR assays for the detection of O. tsutsugamushi and Rickettsia spp. had high specificity (99.4% [95% confidence interval (CI): 96.8-100] and 100%[95%CI: 97.8-100], respectively). The PCR results were also compared with immunoglobulinM(IgM) immunofluorescence assay (IFA) on acute sera. For O. tsutsugamushi, PCR sensitivity was twice that of acute specimen IgM IFA (28.6% versus 14.3%; McNemar's P = 0.3). For Rickettsia spp., PCR was four times as sensitive as acute specimen IgM IFA (36.4% versus 9.1%; P = 0.08), although this was not statistically significant. Whole blood and buffy coat, but not serum, were acceptable specimens for these PCRs. Further evaluation of these assays in a larger prospective study is warranted. Copyright © 2013 by The American Society of Tropical Medicine and Hygiene.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84881513987&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/31894
ISSN: 00029637
Appears in Collections:Scopus 2011-2015

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.