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Title: A serine-to-asparagine mutation at position 314 of H5N1 avian influenza virus NP is a temperature-sensitive mutation that interferes with nuclear localization of NP
Authors: Nattamon Siboonnan
Wittawat Wiriyarat
Chompunuch Boonarkart
Warunya Chakritbudsabong
Anan Jongkaewwattana
Pilaipan Puthavathana
Prasert Auewarakul
Ornpreya Suptawiwat
Mahidol University
National Center of Genetic Engineering and Biotechnology
Keywords: Immunology and Microbiology
Issue Date: 1-Jun-2013
Citation: Archives of Virology. Vol.158, No.6 (2013), 1151-1157
Abstract: We have generated a temperature-sensitive (ts) mutant from a human isolate of the H5N1 avian influenza virus by classical adaptation in cell culture. After 20 passages at low temperature, the virus showed a ts phenotype. The ts mutant also showed an attenuated phenotype after nasal inoculation in mice. Using reverse genetics, we generated reassortants carrying individual genomic segments of the wild-type and mutant viruses in an A/Puerto Rico/8/34 background, and found that the nucleoprotein (NP) gene could confer the ts phenotype. This mutant NP contains a serine-to-asparagine mutation at position 314 (S314N). The mutant NP protein showed a defect in nuclear localization at high temperature in mammalian cells. © 2013 Springer-Verlag Wien.
ISSN: 03048608
Appears in Collections:Scopus 2011-2015

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