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Title: Anti-Pfs25 human plasma reduces transmission of plasmodium falciparum isolates that have diverse genetic backgrounds
Authors: Dari F. Da
Saurabh Dixit
Jetsumon Sattabonkot
Jianbing Mu
Luc Abate
Bhanumati Ramineni
Jean Bosco Ouedraogo
Nicholas J. MacDonald
Michael P. Fay
Xin zhuan Su
Anna Cohuet
Yimin Wu
Institut de Recherche en Sciences de la Santé (IRSS)
Maladies Infectieuses et Vecteurs : Ecologie, Genetique, Evolution et Controle
National Institute of Allergy and Infectious Diseases
Armed Forces Research Institute of Medical Sciences, Thailand
Mahidol University
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Jun-2013
Citation: Infection and Immunity. Vol.81, No.6 (2013), 1984-1989
Abstract: Pfs25 is a leading candidate for a malaria transmission-blocking vaccine whose potential has been demonstrated in a phase 1 trial with recombinant Pfs25 formulated with Montanide ISA51. Because of limited sequence polymorphism, the anti-Pfs25 antibodies induced by this vaccine are likely to have transmission-blocking or -reducing activity against most, if not all, field isolates. To test this hypothesis, we evaluated transmission-blocking activities by membrane feeding assay of anti-Pfs25 plasma from the Pfs25/ISA51 phase 1 trial against Plasmodium falciparum parasites from patients in two different geographical regions of the world, Thailand and Burkina Faso. In parallel, parasite isolates from these patients were sequenced for the Pfs25 gene and genotyped for seven microsatellites. The results indicate that despite different genetic backgrounds among parasite isolates, the Pfs25 sequences are highly conserved, with a single nonsynonymous nucleotide polymorphism detected in 1 of 41 patients in Thailand and Burkina Faso. The anti-Pfs25 immune plasma had significantly higher transmission-reducing activity against parasite isolates from the two geographical regions than the nonimmune controls (P<0.0001). © 2013, American Society for Microbiology.
ISSN: 10985522
Appears in Collections:Scopus 2011-2015

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