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Title: Molecular identification of Paragonimus species by DNA pyrosequencing technology
Authors: Chairat Tantrawatpan
Pewpan M. Intapan
Penchom Janwan
Oranuch Sanpool
Viraphong Lulitanond
Chutatip Srichantaratsamee
Witthaya Anamnart
Wanchai Maleewong
Khon Kaen University
Faculty of Medicine, Thammasat University
Mahidol University
Walailak University
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Jun-2013
Citation: Parasitology International. Vol.62, No.3 (2013), 341-345
Abstract: DNA pyrosequencing for PCR amplicons is an attractive strategy for the identification of microorganisms because of its short time performance for large number of samples. In this study, the primers targeting the fragment of ITS2 region of nuclear ribosomal RNA gene were newly developed for pyrosequencing-based identification of 6 Paragonimus species, Paragonimus bangkokensis, Paragonimus harinasutai, Paragonimus heterotremus, Paragonimus macrorchis, Paragonimus siamensis and Paragonimus westermani. Pyrosequencing determination of 39 nucleotides of partial ITS2 region could discriminate 6 Paragonimus species, and could also detect intra-species genetic variation of P. macrorchis. This DNA pyrosequencing-based identification can be a valuable tool to improve species-level identification of Paragonimus in the endemic areas. © 2012 Elsevier Ireland Ltd.
ISSN: 18730329
Appears in Collections:Scopus 2011-2015

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