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Title: Inter- and intra-operator variability in the reading of indirect immunofluorescence assays for the serological diagnosis of scrub typhus and murine typhus
Authors: Rattanaphone Phetsouvanh
Thaksinaporn Thojaikong
Phonlavanh Phoumin
Bountoy Sibounheuang
Koukeo Phommasone
Vilada Chansamouth
Sue J. Lee
Paul N. Newton
Stuart D. Blacksell
Mahosot Hospital
Mahidol University
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-May-2013
Citation: American Journal of Tropical Medicine and Hygiene. Vol.88, No.5 (2013), 932-936
Abstract: Inter- and intra-observer variation was examined among six microscopists who read 50 scrub typhus (ST) and murine typhus (MT) indirect immunofluorescence assay (IFA) immunoglobulinM(IgM) slides. Inter-observer agreement was moderate (κ = 0.45) for MT and fair (κ = 0.32) for ST, and was significantly correlated with experience (P = 0.03 and P = 0.004, respectively); k-scores for intra-observer agreement between morning and afternoon readings (range = 0.35-0.86) were not correlated between years of experience for ST and MT IFAs (Spearman's ρ = 0.31, P = 0.54 and P = 0.14, respectively; P = 0.78). Storage at 4°C for 2 days showed a change from positive to negative in 20-32% of slides. Although the titers did not dramatically change after 14 days of storage, the final interpretation (positive to negative) did change in 36-50% of samples, and it, therefore, recommended that slides should be read as soon as possible after processing. Copyright © 2013 by The American Society of Tropical Medicine and Hygiene.
ISSN: 00029637
Appears in Collections:Scopus 2011-2015

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