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Title: Potential function of granulysin, other related effector molecules and lymphocyte subsets in patients with TB and HIV/TB coinfection
Authors: Nada Pitabut
Shinsaku Sakurada
Takahiro Tanaka
Chutharut Ridruechai
Junko Tanuma
Takahiro Aoki
Pacharee Kantipong
Surachai Piyaworawong
Nobuyuki Kobayashi
Panadda Dhepakson
Hideki Yanai
Norio Yamada
Shinichi Oka
Masaji Okada
Srisin Khusmith
Naoto Keicho
Mahidol University
National Center for Global Health and Medicine
Chiang Rai Regional Hospital
Mae Chang District Hospital
Thailand Ministry of Public Health
Japan Anti-Tuberculosis Association
The Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association
National Hospital Organization, Japan
Keywords: Medicine
Issue Date: 28-Jun-2013
Citation: International Journal of Medical Sciences. Vol.10, No.8 (2013), 1003-1014
Abstract: Background: Host effector mechanism against Mycobacterium tuberculosis (Mtb) infection is dependent on innate immune response by macrophages and neutrophils and the alterations in balanced adaptive immunity. Coordinated release of cytolytic effector molecules from NK cells and effector T cells and the subsequent granule-associated killing of infected cells have been documented; however, their role in clinical tuberculosis (TB) is still controversy. Objective: To investigate whether circulating granulysin and other effector molecules are associated with the number of NK cells, /NKT cells, Vy9+VÕ2+T cells, CD4+T cells and CD8+T cells, and such association influences the clinical outcome of the disease in patients with pulmonary TB and HIV/TB coinfection. Methods: Circulating granulysin, perforin, granzyme-B and IFN-y levels were determined by ELISA. The isoforms of granulysin were analyzed by Western blot analysis. The effector cells were analyzed by flow cytometry. Results: Circulating granulysin and perforin levels in TB patients were lower than healthy controls, whereas the granulysin levels in HIV/TB coinfection were much higher than in any other groups, TB and HIV with or without receiving HAART, which corresponded to the number of CD8+T cells which kept high, but not with NK cells and other possible cellular sources of granulysin. In addition, the 17kDa, 15kDa and 9kDa isoforms of granulysin were recognized in plasma of HIV/TB coinfection. Increased granulysin and decreased IFN-y levels in HIV/TB coinfection and TB after completion of anti-TB therapy were observed. Conclusion: The results suggested that the alteration of circulating granulysin has potential function in host immune response against TB and HIV/TB coinfection. This is the first demonstration so far of granulysin in HIV/TB coinfection. © Ivyspring International Publisher.
ISSN: 14491907
Appears in Collections:Scopus 2011-2015

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