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Title: Cloning and application of recombinant dengue virus prM-M protein for serodiagnosis of dengue virus infection
Authors: Sineewanlaya Wichit
Hatairat Yongpradoem
Pornapat Surasombatpattana
Pornsawan Leaungwuttiwong
Thareerat Kalambaheti
Wipawee Jampangern
Akanitt Jittmittraphap
Mahidol University
Armed Forces Research Institute of Medical Sciences, Thailand
Keywords: Medicine
Issue Date: 22-May-2013
Citation: Southeast Asian Journal of Tropical Medicine and Public Health. Vol.44, No.2 (2013), 218
Abstract: We studied the use of the precursor to the M structural protein (prM) found only on the surface of mature dengue virus as a target protein to detect dengue virus infection. Recombinant D2-16681 prM-M protein was constructed and tested for immunogenicity with dengue and Japanese encephalitis patient sera by Western blot analysis and indirect ELISA. The sensitivity and specificity of indirect ELISA were 48.1 and 85.5%, respectively, and Western blot assay were 23.1 and 98.7%, respectively, for detection of dengue virus. Although the sensitivity of the indirect ELISA is low, the indirect ELISA using recombinant D2-16681 prM-M proteins as antigen may be used for early detection of dengue virus infection.
ISSN: 01251562
Appears in Collections:Scopus 2011-2015

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