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Title: Performance comparison of immunodiffusion, enzyme-linked immunosorbent assay, immunochromatography and hemagglutination for serodiagnosis of human pythiosis
Authors: Takol Chareonsirisuthigul
Rommanee Khositnithikul
Akarin Intaramat
Ruchuros Inkomlue
Kanchana Sriwanichrak
Savittree Piromsontikorn
Sureewan Kitiwanwanich
Tassanee Lowhnoo
Wanta Yingyong
Angkana Chaiprasert
Ramrada Banyong
Kavi Ratanabanangkoon
Tristan T. Brandhorst
Theerapong Krajaejun
Mahidol University
Chulabhorn Research Institute
University of Wisconsin School of Medicine and Public Health
Keywords: Medicine
Issue Date: 1-May-2013
Citation: Diagnostic Microbiology and Infectious Disease. Vol.76, No.1 (2013), 42-45
Abstract: Pythiosis is a life-threatening infectious disease caused by the fungus-like organism Pythium insidiosum. Morbidity and mortality rates of pythiosis are high. The treatment of choice for pythiosis is surgical debridement of infected tissue. Early and accurate diagnosis is critical for effective treatment. In-house serodiagnostic tests, including immunodiffusion (ID), enzyme-linked immunosorbent assay (ELISA), immunochromatography (ICT) and hemagglutination (HA) have been developed to detect antibodies against P. insidiosum in sera. This study compares the diagnostic performance of ID, ELISA, ICT, and HA, using sera from 37 pythiosis patients and 248 control subjects. ICT and ELISA showed optimal diagnostic performance (100% sensitivity, specificity, positive predictive value and negative predictive value). ICT was both rapid and user-friendly. ELISA results were readily quantitated. ID is relatively insensitive. HA was rapid, but diagnostic performance was poor. Understanding the advantages offered by each assay facilitates selection of an assay that is circumstance-appropriate. This will promote earlier diagnoses and improved outcomes for patients with pythiosis. © 2013 Elsevier Inc.
ISSN: 18790070
Appears in Collections:Scopus 2011-2015

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