Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/33050
Title: Knockdown of a novel G-protein pathway suppressor 2 (GPS2) leads to shrimp mortality by exuvial entrapment during ecdysis
Authors: Pakkakul Sangsuriya
Kornsunee Phiwsaiya
Benjamart Pratoomthai
Thanawat Sriphaijit
Piti Amparyup
Boonsirm Withyachumnarnkul
Saengchan Senapin
Chulalongkorn University
Mahidol University
Thailand National Center for Genetic Engineering and Biotechnology
Rangsit University
Shrimp Genetic Improvement Center
Keywords: Agricultural and Biological Sciences;Environmental Science
Issue Date: 1-Mar-2014
Citation: Fish and Shellfish Immunology. Vol.37, No.1 (2014), 46-52
Abstract: A novel G-protein pathway suppressor 2 (GPS2) has been identified from hemocytes of the whiteleg shrimp Penaeus vannamei (Pv) and appears to play a role in ecdysis. The full-length of PvGPS2 cDNA consisted of a 1230-bp open reading frame, encoding 409 deduced amino acids with significant sequence homology to GPS2 sequences of crustaceans and insects. RT-PCR revealed that PvGPS2 was expressed in all P.vannamei tissues examined, but that expression was molt stage specific in eyestalk tissue. Relative expression was higher in the period before molting (i.e., intermolt and pre-molt stages) than in the post-molt stage. When double-stranded RNA (dsRNA)-mediated RNA interference was employed to inhibit PvGPS2 formation in shrimp, it led to significant mortality due to unsuccessful separation of new cuticle from old cuticle (exuvial entrapment) during ecdysis. © 2014 Elsevier Ltd.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84893472820&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/33050
ISSN: 10959947
10504648
Appears in Collections:Scopus 2011-2015

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