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Title: Mutagenic effects of heavy-ion beam irradiation on in vitro nodal segments of Artemisia annua L.
Authors: Phithak Inthima
Masahiro Otani
Tomoya Hirano
Yoriko Hayashi
Tomoko Abe
Masaru Nakano
Kanyaratt Supaibulwatana
Mahidol University
Niigata University
Keywords: Agricultural and Biological Sciences
Issue Date: 1-Jan-2014
Citation: Plant Cell, Tissue and Organ Culture. Vol.119, No.1 (2014), 131-139
Abstract: © 2014, Springer Science+Business Media Dordrecht. Artemisia annua L. is a commercial source of artemisinin. Nevertheless, artemisinin content within the plant is relatively low and varies depending on genotype and environment. To broaden the genetic variability, the mutation effect of12C-ion beam irradiation on A. annua was examined. Irradiation at 2.5 Gy had a slight lethal effect to nodal segments while a noticeable lethal effect was observed at 5 and 10 Gy. Furthermore, at higher doses (20 and 50 Gy), a severe lethal effect was observed. Mutations at the DNA level of axillary bud-derived shoots were performed by RAPD. The mutation frequency at 10 Gy was about 1.7 and 2.1 times higher than that at 2.5 and 5 Gy, respectively. After growth and artemisinin production observation of 72 irradiated mutants, around 14 and 7 % of them showed higher artemisinin content and artemisinin yield compared to the controls, respectively. The highest artemisinin content in a mutant was 1.43 % DW, which was 3.2-fold higher than the original wild type. Additionally, the highest artemisinin yield in mutants was 3.68 mg/plant, which was around 1.4-fold higher than in the wild type. Moreover, irradiated mutants exhibited antibacterial activity against S. aureus, but the wild types did not. This study presents an effective application of heavy ion beam irradiation to create variations and improve artemisinin production in A. annua.
ISSN: 01676857
Appears in Collections:Scopus 2011-2015

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