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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/33117
Title: HPTLC simultaneous quantification of triterpene acids for quality control of Plantago major L. and evaluation of their cytotoxic and antioxidant activities
Authors: Kartini
S. Piyaviriyakul
P. Siripong
O. Vallisuta
Mahidol University
Universitas Surabaya
National Cancer Institute Thailand
Keywords: Agricultural and Biological Sciences
Issue Date: 1-Jan-2014
Citation: Industrial Crops and Products. Vol.60, (2014), 239-246
Abstract: A validated high performance thin-layer chromatography (HPTLC) method was developed for simultaneous determination of ursolic acid (UA) and oleanolic acid (OA) contents in Plantago major which were collected from several plantation areas in Indonesia. The cytotoxic effect against two cancer cell lines, SiHa and Hep G2, and antioxidant activity were evaluated using the MTT and DPPH-radical scavenging assay, respectively. The test samples included various extracts of P. major from different plant parts using methanol and water as extracting solvents and pure compounds derived from this plant. The results showed that both plant parts and extracting solvents affected the chemical contents and their biological activities. The contents of UA and OA varied according to the organs and provenances of plant. The highest content of UA (0.22-0.48% dry weight) and OA (0.17-0.33% dry weight) were found in the methanol extract of seed. This extract also exhibited the highest cytotoxic activity (IC50value: 174.42-246.38μg/ml), whereas the strongest free radical scavenging activity was obtained from the leaf methanol extract (IC50value: 263.57μg/ml). The developed HPTLC method can be used for routine analysis and standardization of P. major crude drugs, extracts, and/or finished products using UA and OA as appropriate markers for anticancer products. © 2014 Elsevier B.V.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84903850416&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/33117
ISSN: 09266690
Appears in Collections:Scopus 2011-2015

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