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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/33139
Title: Chitosan nanoparticle encapsulated hemagglutinin-split influenza virus mucosal vaccine
Authors: Chompoonuch Sawaengsak
Yasuko Mori
Koichi Yamanishi
Ampol Mitrevej
Nuttanan Sinchaipanid
Mahidol University
Kobe University School of Medicine
National Institute of Biomedical Innovation
Keywords: Agricultural and Biological Sciences;Pharmacology, Toxicology and Pharmaceutics
Issue Date: 1-Jan-2014
Citation: AAPS PharmSciTech. Vol.15, No.2 (2014), 317-325
Abstract: Subunit/split influenza vaccines are less reactogenic compared with the whole virus vaccines. However, their immunogenicity is relatively low and thus required proper adjuvant and/or delivery vehicle for immunogenicity enhancement. Influenza vaccines administered intramuscularly induce minimum, if any, mucosal immunity at the respiratory mucosa which is the prime site of the infection. In this study, chitosan (CS) nanoparticles were prepared by ionic cross-linking of the CS with sodium tripolyphosphate (TPP) at the CS/TPP ratio of 1:0.6 using 2 h mixing time. The CS/TPP nanoparticles were used as delivery vehicle of an intranasal influenza vaccine made of hemagglutinin (HA)-split influenza virus product. Innocuousness, immunogenicity, and protective efficacy of the CS/TPP-HA vaccine were tested in influenza mouse model in comparison with the antigen alone vaccine. The CS/TPP-HA nanoparticles had required characteristics including nano-sizes, positive charges, and high antigen encapsulation efficiency. Mice that received two doses of the CS/TPP-HA vaccine intranasally showed no adverse symptoms indicating the vaccine innocuousness. The animals developed higher systemic and mucosal antibody responses than vaccine made of the HA-split influenza virus alone. The CS/TPP-HA vaccine could induce also a cell-mediated immune response shown as high numbers of IFN-γ-secreting cells in spleens while the HA vaccine alone could not. Besides, the CS nanoparticle encapsulated HA-split vaccine reduced markedly the influenza morbidity and also conferred 100% protective rate to the vaccinated mice against lethal influenza virus challenge. Overall results indicated that the CS nanoparticles invented in this study is an effective and safe delivery vehicle/adjuvant for the influenza vaccine. © 2013 American Association of Pharmaceutical Scientists.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84898010025&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/33139
ISSN: 15309932
Appears in Collections:Scopus 2011-2015

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