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Title: Identification of laticifer-specific genes and their promoter regions from a natural rubber producing plant Hevea brasiliensis
Authors: Yuichi Aoki
Seiji Takahashi
Daisuke Takayama
Yoshiyuki Ogata
Nozomu Sakurai
Hideyuki Suzuki
Kasem Asawatreratanakul
Dhirayos Wititsuwannakul
Rapepun Wititsuwannakul
Daisuke Shibata
Tanetoshi Koyama
Toru Nakayama
Tohoku University
Kazusa DNA Research Institute
Thaksin University
Mahidol University
Prince of Songkla University
Osaka Prefecture University
Keywords: Agricultural and Biological Sciences;Biochemistry, Genetics and Molecular Biology
Issue Date: 1-Jan-2014
Citation: Plant Science. Vol.225, (2014), 1-8
Abstract: Latex, the milky cytoplasm of highly differentiated cells called laticifers, from Hevea brasiliensis is a key source of commercial natural rubber production. One way to enhance natural rubber production would be to express genes involved in natural rubber biosynthesis by a laticifer-specific overexpression system. As a first step to identify promoters which could regulate the laticifer-specific expression, we identified random clones from a cDNA library of H. brasiliensis latex, resulting in 4325 expressed sequence tags (ESTs) assembled into 1308 unigenes (692 contigs and 617 singletons). Quantitative analyses of the transcription levels of high redundancy clones in the ESTs revealed genes highly and predominantly expressed in laticifers, such as Rubber Elongation Factor (REF), Small Rubber Particle Protein and putative protease inhibitor proteins. HRT1 and HRT2, cis-prenyltransferases involved in rubber biosynthesis, was also expressed predominantly in laticifers, although these transcript levels were 80-fold lower than that of REF. The 5'-upstream regions of these laticifer-specific genes were cloned and analyzed in silico, revealing seven common motifs consisting of eight bases. Furthermore, transcription factors specifically expressed in laticifers were also identified. The common motifs in the laticifer-specific genes and the laticifer-specific transcription factors are potentially involved in the regulation of gene expression in laticifers. © 2014 Elsevier Ireland Ltd.
ISSN: 01689452
Appears in Collections:Scopus 2011-2015

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