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Title: Cytoprotection by the NO-donor SNAP against ischemia/reoxygenation injury in mouse embryonic stem cell-derived cardiomyocytes
Authors: A. Görbe
Z. V. Varga
J. Pálóczi
S. Rungarunlert
N. Klincumhom
M. K. Pirity
R. Madonna
T. Eschenhagen
A. Dinnyés
T. Csont
P. Ferdinandy
Cardiovascular Research Group, Szeged
Chulalongkorn University
Szent Istvan Egyetem
Universitatsklinikum Hamburg-Eppendorf und Medizinische Fakultat
BioTalentum Ltd.
Texas Heart Institute
Semmelweis Egyetem
Pharmahungary Group
Biological Research Center at Hungarian Academy of Sciences
University of G. d'Annunzio Chieti and Pescara
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology;Chemical Engineering;Immunology and Microbiology
Issue Date: 1-Mar-2014
Citation: Molecular Biotechnology. Vol.56, No.3 (2014), 258-264
Abstract: Embryonic stem cell (ESC)-derived cardiomyocytes are a promising cell source for the screening for potential cytoprotective molecules against ischemia/reperfusion injury, however, little is known on their behavior in hypoxia/reoxygenation conditions. Here we tested the cytoprotective effect of the NO-donor SNAP and its downstream cellular pathway. Mouse ESC-derived cardiomyocytes were subjected to 150-min simulated ischemia (SI) followed by 120-min reoxygenation or corresponding non-ischemic conditions. The following treatments were applied during SI or normoxia: the NO-donor S-Nitroso-N-acetyl- d,l-penicillamine (SNAP), the protein kinase G (PKG) inhibitor, the K ATP channel blocker glibenclamide, the particulate guanylate cyclase activator brain type natriuretic peptide (BNP), and a non-specific NO synthase inhibitor (N-Nitro-l-arginine, l-NNA) alone or in different combinations. Viability of cells was assayed by propidium iodide staining. SNAP attenuated SI-induced cell death in a concentration-dependent manner, and this protection was attenuated by inhibition of either PKG or KATP channels. However, SI-induced cell death was not affected by BNP or by l-NNA. We conclude that SNAP protects mESC-derived cardiomyocytes against SI/R injury and that soluble guanylate-cyclase, PKG, and KATP channels play a role in the downstream pathway of SNAP-induced cytoprotection. The present mESC-derived cardiomyocyte-based screening platform is a useful tool for discovery of cytoprotective molecules. © 2013 Springer Science+Business Media New York.
ISSN: 15590305
Appears in Collections:Scopus 2011-2015

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