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Title: Estrogen reduces endoplasmic reticulum stress to protect against glucotoxicity induced-pancreatic β-cell death
Authors: Suwattanee Kooptiwut
Pitchnischa Mahawong
Wanthanee Hanchang
Namoiy Semprasert
Suchada Kaewin
Thawornchai Limjindaporn
Pa Thai Yenchitsomanus
Mahidol University
Faculty of Medicine, Siriraj Hospital, Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology;Medicine
Issue Date: 1-Jan-2014
Citation: Journal of Steroid Biochemistry and Molecular Biology. Vol.139, (2014), 25-32
Abstract: Estrogen can improve glucose homeostasis not only in diabetic rodents but also in humans. However, the molecular mechanism by which estrogen prevents pancreatic β-cell death remains unclear. To investigate this issue, INS-1 cells, a rat insulinoma cell line, were cultured in medium with either 11.1 mM or 40 mM glucose in the presence or the absence of estrogen. Estrogen significantly reduced apoptotic β-cell death by decreasing nitrogen-induced oxidative stress and the expression of the ER stress markers GRP 78, ATF6, P-PERK, PERK, uXBP1, sXBP1, and CHOP in INS-1 cells after prolonged culture in medium with 40 mM glucose. In contrast, estrogen increased the expression of survival proteins, including sarco/endoplasmic reticulum Ca2+ATPase (SERCA-2), Bcl-2, and P-p38, in INS-1 cells after prolonged culture in medium with 40 mM glucose. The cytoprotective effect of estrogen was attenuated by addition of the estrogen receptor (ERα and ERβ) antagonist ICI 182,780 and the estrogen membrane receptor inhibitor G15. We showed that estrogen decreases not only oxidative stress but also ER stress to protect against 40 mM glucose-induced pancreatic β-cell death. © 2013 Elsevier Ltd. All rights reserved.
ISSN: 18791220
Appears in Collections:Scopus 2011-2015

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