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Title: Rapid and sensitive lateral flow immunoassay for influenza antigen using fluorescently-doped silica nanoparticles
Authors: Suwussa Bamrungsap
Chayachon Apiwat
Warangkana Chantima
Tararaj Dharakul
Natpapas Wiriyachaiporn
Thailand National Science and Technology Development Agency
Mahidol University
Keywords: Chemistry
Issue Date: 1-Jan-2014
Citation: Microchimica Acta. Vol.181, No.1-2 (2014), 223-230
Abstract: We report on a lateral flow immunoassay (LFIA) for influenza A antigen using fluorescently-doped silica nanoparticles as reporters. The method is taking advantage of the high brightness and photostability of silica nanoparticles (doped with the dye Cy5) and the simplicity and rapidity of LFIA. The nucleoprotein of influenza A virion (one of its most abundant structural proteins) was used as a model to demonstrate a performance of the LFIA. Under optimized conditions and by using a portable strip reader, the fluorescence-based LFIA is capable of detecting a recombinant nucleoprotein as low as 250 ng · mL-1 using a sample volume of 100 μL, within 30 min, and without interference by other proteins. The successful detection of the nucleoprotein in infected allantoic fluid demonstrated the functionality of the method. By comparison with a commercial influenza A test based on gold nanoparticles as reporters, the system provides an 8-fold better sensitivity. [Figure not available: see fulltext.] © 2013 Springer-Verlag Wien.
ISSN: 14365073
Appears in Collections:Scopus 2011-2015

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