Simple jQuery Dropdowns
Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/33925
Title: Ophiophagus hannah venom: Proteome, components bound by Naja kaouthia antivenin and neutralization by n. kaouthia neurotoxin-specific human ScFv
Authors: Witchuda Danpaiboon
Onrapak Reamtong
Nitat Sookrung
Watee Seesuay
Yuwaporn Sakolvaree
Jeeraphong Thanongsaksrikul
Fonthip Dong-din-on
Potjanee Srimanote
Kanyarat Thueng-In
Wanpen Chaicumpa
Mahidol University
Thammasat University
Kasetsart University
Keywords: Environmental Science;Pharmacology, Toxicology and Pharmaceutics
Issue Date: 1-Jan-2014
Citation: Toxins. Vol.6, No.5 (2014), 1526-1558
Abstract: Venomous snakebites are an important health problem in tropical and subtropical countries. King cobra (Ophiophagus hannah) is the largest venomous snake found in South and Southeast Asia. In this study, the O. hannah venom proteome and thevenom components cross-reactive to N. kaouthia monospecific antivenin were studied. O. hannah venom consisted of 14 different protein families, including three finger toxins, phospholipases, cysteine-rich secretory proteins, cobra venom factor, muscarinic toxin, L-amino acid oxidase, hypothetical proteins, low cysteine protein, phosphodiesterase, proteases, vespryn toxin, Kunitz, growth factor activators and others (coagulation factor, endonuclease, 5'-nucleotidase). N. kaouthia antivenin recognized several functionally different O. hannah venom proteins and mediated paratherapeutic efficacy by rescuing the O. hannah envenomed mice from lethality. An engineered human ScFv specific to N. kaouthia long neurotoxin (NkLN-HuScFv) cross-neutralized the O. hannah venom and extricated the O. hannah envenomed mice from death in a dose escalation manner. Homology modeling and molecular docking revealed that NkLN-HuScFv interacted with residues in loops 2 and 3 of the neurotoxins of both snake species, which are important for neuronal acetylcholine receptor binding. The data of this study are useful for snakebite treatment when and where the polyspecific antivenin is not available. Because the supply of horse-derived antivenin is limited and the preparation may cause some adverse effects in recipients, a cocktail of recombinant human ScFvs for various toxic venom components shared by different venomous snakes, exemplified by the in vitro produced NkLN-HuScFv in this study, should contribute to a possible future route for an improved alternative to the antivenins. © 2014 by the authors; licensee MDPI, Basel, Switzerland.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84901236392&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/33925
ISSN: 20726651
Appears in Collections:Scopus 2011-2015

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.