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dc.contributor.authorPeipei Lien_US
dc.contributor.authorZhenjun Zhaoen_US
dc.contributor.authorYing Wangen_US
dc.contributor.authorHua Xingen_US
dc.contributor.authorDaniel M. Parkeren_US
dc.contributor.authorZhaoqing Yangen_US
dc.contributor.authorElizabeth Baumen_US
dc.contributor.authorWenli Lien_US
dc.contributor.authorJetsumon Sattabongkoten_US
dc.contributor.authorJeeraphat Sirichaisinthopen_US
dc.contributor.authorShuying Lien_US
dc.contributor.authorGuiyun Yanen_US
dc.contributor.authorLiwang Cuien_US
dc.contributor.authorQi Fanen_US
dc.contributor.otherDalian Institute of Biotechnologyen_US
dc.contributor.otherThird Military Medical Universityen_US
dc.contributor.otherDalian University of Technologyen_US
dc.contributor.otherPennsylvania State Universityen_US
dc.contributor.otherKunming Medical Universityen_US
dc.contributor.otherUniversity of California, Irvineen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherVector Borne Disease Training Centeren_US
dc.identifier.citationMalaria Journal. Vol.13, No.1 (2014)en_US
dc.description.abstractBackground: Nested PCR is considered a sensitive and specific method for detecting malaria parasites and is especially useful in epidemiological surveys. However, the preparation of DNA templates for PCR is often time-consuming and costly. Methods. A simplified PCR method was developed to directly use a small blood filter paper square (2 × 2 mm) as the DNA template after treatment with saponin. This filter paper-based nested PCR method (FP-PCR) was compared to microscopy and standard nested PCR with DNA extracted by using a Qiagen DNA mini kit from filter paper blood spots of 204 febrile cases. The FP-PCR technique was further applied to evaluate malaria infections in 1,708 participants from cross-sectional epidemiological surveys conducted in Myanmar and Thailand. Results: The FP-PCR method had a detection limit of ∼0.2 parasites/μL blood, estimated using cultured Plasmodium falciparum parasites. With 204 field samples, the sensitivity of the FP-PCR method was comparable to that of the standard nested PCR method, which was significantly higher than that of microscopy. Application of the FP-PCR method in large cross-sectional studies conducted in Myanmar and Thailand detected 1.9% (12/638) and 6.2% (66/1,070) asymptomatic Plasmodium infections, respectively, as compared to the detection rates of 1.3% (8/638) and 0.04% (4/1,070) by microscopy. Conclusion: This FP-PCR method was much more sensitive than microscopy in detecting Plasmodium infections. It drastically increased the detection sensitivity of asymptomatic infections in cross-sectional surveys conducted in Thailand and Myanmar, suggesting that this FP-PCR method has a potential for future applications in malaria epidemiology studies. © 2014 Li et al.; licensee BioMed Central Ltd.en_US
dc.rightsMahidol Universityen_US
dc.subjectImmunology and Microbiologyen_US
dc.titleNested PCR detection of malaria directly using blood filter paper samples from epidemiological surveysen_US
Appears in Collections:Scopus 2011-2015

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