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Title: Diagnosis of gnathostomiasis by skin testing using partially purified specific antigen and total IgE levels
Authors: Wirach Maek-a-nantawat
Valai Bussaratid
Benjaluck Phonrat
Wallop Pakdee
Supaporn Nuamtanong
Paron Dekumyoy
Mahidol University
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Feb-2014
Citation: Transactions of the Royal Society of Tropical Medicine and Hygiene. Vol.108, No.2 (2014), 71-76
Abstract: Background: A finding of antibodies to Gnathostoma spinigerum 24-kDa antigen by immunoblot analysis is currently used to confirm a diagnosis of gnathostomiasis. A simple skin test for the diagnosis of gnathostomiasis was developed, and the results were evaluated and compared with the standard Western blot (WB) test. Methods: This cross-sectional study was conducted at the Hospital for Tropical Diseases, Bangkok, Thailand, in 2008-2011. All eligible patients were tested with partially purified proteins of mAb-detected fractions pooled and sterilized by 0.2 mm diameter syringe filter, with a phenol saline solution of 1:10 w/v. Results: A total of 69 cases, 39 gnathostomiasis cases and 30 controls, were enrolled into the study; the median age (IQR) was 40 (30.5-52.5) years. The most common presenting symptom was edema (56/69, 81%). Gnathostomiasis cases having strong cutaneous reactions to the intradermal test (81%) were also positive by immunoblot. A significant correlation between skin and immunoblot tests was detected (p<0.001). The difference in total IgE levels between cases and controls was not statistically significant (p=0.51). Logistic regression models showed that positive WB and skin-test results were significantly associated with gnathostomiasis (p=0.001 and p=0.007, respectively). Conclusion: Gnathostoma skin testing, using prepared fractionated antigen solution of Gnathostoma spinigerum, yields good reactivity and significantly correlates with the results of immunoblot testing. © The Author 2013. Published by Oxford University Press on behalf of Royal Society of Tropical Medicine and Hygiene. All rights reserved.
ISSN: 18783503
Appears in Collections:Scopus 2011-2015

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