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Title: Evaluation of chimeric DNA vaccines consisting of premembrane and envelope genes of Japanese encephalitis and dengue viruses as a strategy for reducing induction of dengue virus infection-enhancing antibody response
Authors: Fithriyah Sjatha
Miwa Kuwahara
T. Mirawati Sudiro
Masanori Kameoka
Eiji Konishi
Kobe University School of Medicine
Universitas Indonesia
Mahidol University
Keywords: Immunology and Microbiology
Issue Date: 1-Feb-2014
Citation: Microbiology and Immunology. Vol.58, No.2 (2014), 126-134
Abstract: Neutralizing antibodies induced by dengue virus (DENV) infection show viral infection-enhancing activities at sub-neutralizing doses. On the other hand, preimmunity against Japanese encephalitis virus (JEV), a congener of DENV, does not increase the severity of DENV infection. Several studies have demonstrated that neutralizing epitopes in the genus Flavivirus are mainly located in domain III (DIII) of the envelope (E) protein. In this study, chimeric premembrane and envelope (prM-E) gene-based expression plasmids of JEV and DENV1 with DIII substitution of each virus were constructed for use as DNA vaccines and their immunogenicity evaluated. Sera from C3H/He and ICR mice immunized with a chimeric gene containing DENV1 DIII on a JEV prM-E gene backbone showed high neutralizing antibody titers with less DENV infection-enhancing activity. Our results confirm the applicability of this approach as a new dengue vaccine development strategy. © 2014 The Societies and Wiley Publishing Asia Pty Ltd.
ISSN: 13480421
Appears in Collections:Scopus 2011-2015

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